Chapter 1

The Scope of Biochemistry


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The following image of a DNA-Depleted Human (HeLa) Cell-Line Chromosome was generated using Electron Microscopy Tomography (EMT).

A movie of the reconstruction viewpoint changing with 3 degree increments around tilt axis can also be viewed.

The following image represents an electron microscope picture of the Uukuniemi virus. The virus was briefly stained with uranylacetate in methanol and freeze-dried from t-butanol before the photography. These pictures were aligned as regards to rotations, translations, and scaling using colloidal (5-10nm diameter) gold particles as reference points (you can see two of the gold particles in the movie below, sphere-like objects colored red).

A volume rendered movie of the reconstruction, viewpoint changing with 3 degree increments around the tilt axis can also be viewed.


Space-Filling Models

The van der Waals radius (R) is defined as the effective radius for closest molecular packing and represents the distance of closest approach for an atom or group of atoms, e.g. H2O. In space-filling models of a molecule each atom or group is represented by a sphere of appropriate radius giving the molecule a compact, space-filled shape. An example is the representation of the enzyme lysozyme.